Iron compound from animal proteids and process of manufacture thereof



Patented Jan. 7, 1930 'NI T-ED "Li-SET PATENT orries A SWIG-EL POSTERNAK AND TI-IEODOR POSTERNAK, 0. 5 :CHENEBOUGERIES, NEAR GENEVA, SWITZERLAND, ASSIGNORS TO SOCIETY OF CHEMICAL INDUSTRY .IN

BASLE, OFBASEL, SWITZERLAND IRON COMPOUND FROM, ANIMAL PROTEIDS AND 'PROCESS OF MANUFACTURE THEREOF xrNoDra'wing. pplication" fiiedfl'anuary 4,1 1928, Serial N 0. 244,549, and in Switzerland January 17, 1927.

,The present invention .relates to new iron compounds ofthe-phosphorus nuclei obtained fromthe-proteids ofeggyolk, said'compounds beinguseful in therapeutics,- for instance as tonics, and it comprises, the new compounds themselves as well as the process of their manufacture.

; In the co-pendingapplication Serial No. 177,220.;a; process is described according to which, by; subjecting-egg yolk in-succession to a peptic andatryptic; digestion, mixtures of the phosphorus nuclei-.ofrthe proteids con- ,tainediin-egg yolk are obtained which may be isolated Il'Il the free :form or. as salts.

.In the-further-co-pendingspecificationSestudy of the two phosphorus nuclei or and [3 :llfiS shown, that they are very similar to each other and differ only by the iron content of the latter. 'lBoth compounds possess, except ;-for the iron; content, the same chemical Composition, yield byih'ydrolysis the same amino 1 acids,.principallyserine, andcontain the same .numberyof; phosphoric: acid, radicals, which arevzesteirified -.with the hydroxyl; groups of serine, two phosphoric acid. radicals being present-in the free form. Both compounds .are thus polybasic acidswhich are-actually 1 capable of bindingtwo equivalents of light imetals. for each-phosphoric acid radical, as

fen-instance alkaline ora-lkaline-earth bases.

7 40 According to this fact itseems-th-at the iron sis notiboundin thesaltiorm in the: phosi-phorusinucleus ,8.

Ithasnow: been found, that the phosphorus D nucleus 0: behaves difierently towards alkalies 1 an d. alkalineearths than towards iron, i. .e. it

binds not only two equivalents for each phosphoric acid radical, as it wasto be expected, but somewhat morethan' 3 equivalents ofiron, whereby those valences are first saturated which have nothing todo with the acid capacity of the compound; asshown bythe' fact that, if the phosphorus nucleus or is precipitated with about 1.2 equivalents of iron'or less, the iron is bound organically,the".free phosphoric acid hydroxyl groups remaining.- intact and being capable of partial or total saturation by. metals, as for instance alkali metals or alkaline earth metals.

The 'ironcompound ofthe phosphorus nucleus ii thus obtained is very similar to" the 2' phosphorus nucleus'fiand differs froInthe latter only by'its diminished'stability towards caustic alkalies. 1 It contains about 5 per cent of iron.

Both the nuclei 0: and ,8- are capable to bind; more iron, thus yielding genuine iron-salts of saturated or acid character.

Theacid, i. e. unsaturated,'iron saltsare formed in presence of an excess of a soluble iron compound in strongly acid solution, 'the 1 saturated salts by treating a neutral, solution a of the phosphorus nuclei with an excessof a soluble iron compound.

The acid salts are whitish-yellow. andcontainabout 14.5,per centof iron aridl0.5..per cent of-phosphorus.

The saturated saltsarereddish-yellow and contain about 18.0 per cent of iron and 9.5

.per cent of; phosphorus.

The -iron compounds ,-:containing 1 only i organically bound iron as well as the acid =iron salts are-capable to react. withisoluble :compounds of-light metalsof the firstandisecond series of the periodicusystem,: for; instance with alkali or F alkaline; earth 1 {metal com- 1 pounds,- thus yielding mixed compounds; of the phosphorus nuclei withsaid .metals and iron,-Which maybe separated out by precipitation with alcohol.

Instead of the isolated;:plirosphoiiis nuclei' v soluble in water and contains about4.5 per also a mixture of them or a raw digestionmixture obtained by successive peptic and tryptic digestion of egg yolk according to the co-.

pending application Serial No. 177 ,220 or No. 177 ,222 may be employed as starting material for the preparation of the above named new iron compounds. H

The invention is illustrated by the following examples Example 1 1 kilogram of phosphorus nucleus .1 is dissolved in Weak soda solution or in very dilute amonia, the solution acidulated with acetic acid, and 200 grams of crystallized iron chloride in aqueous solution added thereto. The mixture is precipitated with concen trated hydrochloric acid, the precipitated filtered, washed and dried. The iron com pound thus obtained contains more than 5 per cent of organically bound iron. 0

For preparing the alkali metal salts of this ironacompound the same is dissolved in'water madealkaline with soda and precipitated with alcohol. After drying the precipitate a yellowish powder is obtained, which is cent of iron and about 10.5 per cent of sodium. In order to obtain the alkaline earth metal salts of the iron compound described inpara- I graph 1 the latter is'dissolved in a small ex cess of ammonia,the solution precipitated "with a solution of calcium chloride, the pre- H cipitate filtered, thoroughly washed with water, dried and pulverized. The iron calcium compound thus obtained forms a white powder, containing 4.5 per cent of iron and about 10 per cent of calcium, and being insoluble in water.

" The mixture obtained is precipitated with an J bound. i 1

Example 2 1 kilogram of araw mixture of the phosphorus nuclei or, B and 7 is dissolvedin weak soda solution and the solution acidulated with acetic acid, and a solution of 500 grams of crystallized iron chloride added thereto.

excess of concentrated hydrochloric acid, filtered and thoroughly washed with water. The dried salt contains about 14.5 per cent of iron, whereof about one half is organically Emample 3 Q 1 kilogram of phosphorus nucleus or or of a mixture of the phosphorus nuclei or and B are dissolved in weak soda solution or in very dilute ammonia, then 520 grams of crystallized iron chloride added thereto and th-ewell stirred mixture treated with an excess of concentrated hydrochloric acid. The precipil tate is filtered, thoroughly washed with water and dried. V The yellowish white-compound thus obtained contains about 14.5. per cent of iron, whereofsomewhat more than one half is organically bound.-

Ewample 4 10 kilogram of defatted egg yolk are subjected first to a peptic digestion in a m neral acid medium. The action of pepsin 1s arrested at a desired stage by total or partial neutralization, the digestion mixture is filtered and the precipitate subjected to a second digestion with trypsin ina weak alkaline; solution. The tryptic digestion liquid thusobtained is exactly neutralized with hydrochloric acid and a solution of 350 grams of crystallized iron chloride added thereto. The precipitate is filtered, thoroughly washed with water, and dried. The salt thus obtained form a reddish-yellow powder and containing about 18.0 per cent of iron.

Example 5' It is obvious that in the above mentioned examples instead of hydrochloric acid other mineral acids, as for instance nitric acid, may be employed for digesting theproteids or for precipitating the iron compounds.

lVhat we claim is r 1 1. Process for obtaining iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, by adding a soluble iron compound to a solution containing at least the first of the three phosphorus nuclei or, B and y; under non-alkaline conditions.

2. Process for obtaining iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, by adding a soluble iron compound to a solution containing at least the first of the three phosphorus nuclei (1, B agd y; in presence of an excess of'a mineral ac1 3. Process for'obtaining iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, by adding a soluble iron compound to a solution containing at least the first of the three phosphorus nuclei a,

B and in presence of an excess of a mineral acid, the quantity of the iron compound being insufficient to saturate the freehydroxyl groups of the phosphorus nuclei.

4. Process for obtaining iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, by adding a soluble iron compound to a solution containing at. least the first of the three phosphorus nuclei or,

B and in presenceof an excess offa mineral acid, the quantity of the iron compound being insuflicient to saturate the free hydroxyl groups of the phosphorus nuclei, dissolving the precipitate formed and treating with alcohol in presence of a solution of a compound of a light metal belonging to one of the two first groups of the periodic system.

5. Process for obtaining iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, by adding a soluble iron compound to a solution containing at least the first of the three phosphorus nuclei 0:, ,8 and in presence of an excess of a mineral acid, the quantity of the iron compound being insuflicient to saturate the free hydroxyl groups of the phosphorus nuclei, dissolving the precipitate formed in a solution of an alkali-compound, and precipitating the solution thus obtained with alcohol.

6. As new products useful in therapeutics iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, said compounds containing the iron at least partly in organic binding and being soluble in soda.

7. As new products useful in therapeutics iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, said compounds containing from about 5 per cent to about 18 per cent of iron, the latter being at least partly present in organic binding, said products being soluble in soda.

8. As new products useful in therapeutics iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, said compounds containing about 5 per cent of iron in organic binding, said products being soluble in soda.

9. As new products useful in therapeutics iron compound of the phospohrus nuclei of the proteids contained in egg yolk, said compounds containing iron and a light metal of one of the first two groups of the periodic system.

10. As new products useful in therapeutics iron compounds of the phosphorus nuclei of the proteids contained in egg yolk, said compounds containing iron and an alkali metal, the iron being present in organic binding, said products being soluble in soda.

In witness whereof we have hereunto signed our names this 21st day of December, 1927.

SWIGEL POSTERNAK. THEODOR POSTERNAK. 

